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Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice
DOI:

07:49 min

June 02, 2022

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Chapters

  • 00:04Introduction
  • 00:48Stereotaxic Surgery
  • 01:54Commercial Customized Guide Cannula Implantation
  • 03:18Infusion Set Up and System Settings of the Microinjection Controller
  • 05:37Infusion of SCFAs into Lateral Ventricle and Validation of Intracerebroventricular Injection
  • 06:29Results: Analysis of SCFAs-Infused Mice
  • 07:09Conclusion

Summary

Automatic Translation

Gut-derived microbial metabolites have multifaceted effects leading to complex behavior in animals. We aim to provide a step-by-step method to delineate the effects of gut-derived microbial metabolites in the brain via intracerebroventricular delivery via a guide cannula.

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