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Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice
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JoVE Journal Neuroscienze
Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice

Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice

DOI:
10.3791/63972-v

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07:49 min

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June 02, 2022

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, ,
1Institute of Basic Medical Sciences, College of Medicine,National Cheng Kung University, 2Department of Physiology, College of Medicine,National Cheng Kung University

Capitoli

  • 00:04Introduction
  • 00:48Stereotaxic Surgery
  • 01:54Commercial Customized Guide Cannula Implantation
  • 03:18Infusion Set Up and System Settings of the Microinjection Controller
  • 05:37Infusion of SCFAs into Lateral Ventricle and Validation of Intracerebroventricular Injection
  • 06:29Results: Analysis of SCFAs-Infused Mice
  • 07:09Conclusion

Summary

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Traduzione automatica

Gut-derived microbial metabolites have multifaceted effects leading to complex behavior in animals. We aim to provide a step-by-step method to delineate the effects of gut-derived microbial metabolites in the brain via intracerebroventricular delivery via a guide cannula.

Tags

Keywords: IntracerebroventricularGut MicrobiotaGut MetabolitesMouse BrainComportamentoStereotaxicGuide CannulaN-Butyl CyanoacrylateDental Acrylic

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