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Demonstration der Sequenzausrichtung zur Vorhersage über Spezies hinweg Suszeptibilitätstool zur schnellen Bewertung der Proteinkonservierung
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Genetics
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JoVE Journal Genetics
Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation
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Demonstration der Sequenzausrichtung zur Vorhersage über Spezies hinweg Suszeptibilitätstool zur schnellen Bewertung der Proteinkonservierung

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2,371 Views

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16:02 min

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February 10, 2023

DOI:

10.3791/63970-v

DOI:
10.3791/63970-v

•

16:02 min
February 10, 2023

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9 Views
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1Office of Research and Development, Center for Computational Toxicology and Exposure, Scientific Computing and Data Curation Division,U.S. Environmental Protection Agency, 2Oak Ridge Institute for Science and Education, 3Swenson College of Science and Engineering, Department of Biology,University of Minnesota Duluth, 4University of Wisconsin-Madison Aquatic Sciences Center, 5General Dynamics Information Technology,Research Triangle Park, 6Office of Research and Development, Center for Computational Toxicology and Exposure, Great Lakes Toxicology and Ecology Division,U.S. Environmental Protection Agency

Summary

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Hier stellen wir ein Protokoll vor, um die neueste Version des SeqAPASS-Tools (Sequence Alignment to Predict Across Species Susceptibility) der US Environmental Protection Agency zu verwenden. Dieses Protokoll demonstriert die Anwendung des Online-Tools zur schnellen Analyse der Proteinkonservierung und zur Bereitstellung anpassbarer und leicht interpretierbarer Vorhersagen der chemischen Empfindlichkeit über Spezies hinweg.

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Vliet, S. M. F., Hazemi, M., Blatz, D., Jensen, M., Mayasich, S., Transue, T. R., Simmons, C., Wilkinson, A., LaLone, C. A. Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation. J. Vis. Exp. (192), e63970, doi:10.3791/63970 (2023).

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