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Visualizing the Morphological Characteristics of Neuromuscular Junction in Rat Medial Gastrocnemius Muscle
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May 17, 2022
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The protocol shows a method to examine spatial correlation among the pre-synaptic terminals, post-synaptic receptors, and peri-synaptic Schwann cells in the rat medial gastrocnemius muscle using fluorescent immunohistochemistry with different biomarkers, namely, neurofilament 200, vesicular acetylcholine transporter, alpha-bungarotoxin, and S100.
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Neuromuscular JunctionMedial Gastrocnemius MuscleRatMorphological CharacteristicsSchwann CellsPre-synapticPost-synapticImmunofluorescencePerfusionCryoprotectionCryosectioningTriton X-100Normal Donkey SerumNeurofilament 200Vesicular Acetylcholine TransporterDonkey Anti-rabbit AF 488Donkey Anti-goat AF 594
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Cui, J., Wu, S., Wang, J., Wang, Y., Su, Y., Xu, D., Liu, Y., Gao, J., Jing, X., Bai, W. Visualizing the Morphological Characteristics of Neuromuscular Junction in Rat Medial Gastrocnemius Muscle. J. Vis. Exp. (183), e63954, doi:10.3791/63954 (2022).
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