/
/
Visualizing the Morphological Characteristics of Neuromuscular Junction in Rat Medial Gastrocnemius Muscle
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Visualizing the Morphological Characteristics of Neuromuscular Junction in Rat Medial Gastrocnemius Muscle
2,846 Views
•
08:42 min
•
May 17, 2022
DOI:
Summary
Automatically generated
The protocol shows a method to examine spatial correlation among the pre-synaptic terminals, post-synaptic receptors, and peri-synaptic Schwann cells in the rat medial gastrocnemius muscle using fluorescent immunohistochemistry with different biomarkers, namely, neurofilament 200, vesicular acetylcholine transporter, alpha-bungarotoxin, and S100.
Explore More Videos
Neuromuscular JunctionMedial Gastrocnemius MuscleRatMorphological CharacteristicsSchwann CellsPre-synapticPost-synapticImmunofluorescencePerfusionCryoprotectionCryosectioningTriton X-100Normal Donkey SerumNeurofilament 200Vesicular Acetylcholine TransporterDonkey Anti-rabbit AF 488Donkey Anti-goat AF 594
Read Article
Cite this Article
Cui, J., Wu, S., Wang, J., Wang, Y., Su, Y., Xu, D., Liu, Y., Gao, J., Jing, X., Bai, W. Visualizing the Morphological Characteristics of Neuromuscular Junction in Rat Medial Gastrocnemius Muscle. J. Vis. Exp. (183), e63954, doi:10.3791/63954 (2022).
Copy