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Neuroscience

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JoVE Journal Neuroscience
Visualizing the Morphological Characteristics of Neuromuscular Junction in Rat Medial Gastrocnemius Muscle
 

Visualizing the Morphological Characteristics of Neuromuscular Junction in Rat Medial Gastrocnemius Muscle

Article DOI: 10.3791/63954-v • 08:42 min • May 17th, 2022
May 17th, 2022 •
*1, *1, 1, 1, 1, 1, 1, 1, 1, 1
1Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences
* These authors contributed equally

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Summary
Automatic Translation

The protocol shows a method to examine spatial correlation among the pre-synaptic terminals, post-synaptic receptors, and peri-synaptic Schwann cells in the rat medial gastrocnemius muscle using fluorescent immunohistochemistry with different biomarkers, namely, neurofilament 200, vesicular acetylcholine transporter, alpha-bungarotoxin, and S100.

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Keywords: Neuromuscular Junction Medial Gastrocnemius Muscle Rat Morphological Characteristics Schwann Cells Pre-synaptic Post-synaptic Immunofluorescence Perfusion Cryoprotection Cryosectioning Triton X-100 Normal Donkey Serum Neurofilament 200 Vesicular Acetylcholine Transporter Donkey Anti-rabbit AF 488 Donkey Anti-goat AF 594
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