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Imaging 3D quantitativo di cellule infette da Trypanosoma cruzi, amastigoti dormienti e cellule T in organi chiarificati intatti
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantitative 3D Imaging of Trypanosoma cruzi-Infected Cells, Dormant Amastigotes, and T Cells in Intact Clarified Organs
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Imaging 3D quantitativo di cellule infette da Trypanosoma cruzi, amastigoti dormienti e cellule T in organi chiarificati intatti

italiano

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2,839 Views

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08:17 min

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June 23, 2022

DOI:

10.3791/63919-v

DOI:
10.3791/63919-v

•

08:17 min
June 23, 2022

•

4 Views
, , , ,
1Center for Tropical and Emerging Global Diseases,University of Georgia, 2Department of Cellular Biology,University of Georgia

Summary

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Il presente protocollo descrive la microscopia fluorescente a foglio luminoso e metodi automatizzati assistiti da software per visualizzare e quantificare con precisione i parassiti proliferanti e dormienti del Trypanosoma cruzi e le cellule T in organi e tessuti intatti e cancellati. Queste tecniche forniscono un modo affidabile per valutare i risultati del trattamento e offrono nuove informazioni sulle interazioni parassita-ospite.

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Cite this Article

Sanchez-Valdez, F., Padilla, Á. M., Bustamante, J. M., Hawkins, C. W. D., Tarleton, R. L. Quantitative 3D Imaging of Trypanosoma cruzi-Infected Cells, Dormant Amastigotes, and T Cells in Intact Clarified Organs. J. Vis. Exp. (184), e63919, doi:10.3791/63919 (2022).

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