/
/
Quantitative 3D Imaging of Trypanosoma cruzi-Infected Cells, Dormant Amastigotes, and T Cells in Intact Clarified Organs
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Quantitative 3D Imaging of Trypanosoma cruzi-Infected Cells, Dormant Amastigotes, and T Cells in Intact Clarified Organs
Capítulos
- 00:04Introduction
- 01:10Tissue Clearing and DNA Staining
- 02:40Immunostaining
- 04:27RI Matching and Mounting
- 05:22Results I: Visualization of Trypanosoma cruzi‐Infected Cells, Dormant Amastigotes, and Inflammation Foci in Cleared Organs
- 06:27Results II: Immunodetection of Vasculature in Cleared Trypanosoma cruzi‐Infected Organs
- 07:07Conclusion
The present protocol describes light-sheet fluorescent microscopy and automated software-assisted methods to visualize and precisely quantify proliferating and dormant Trypanosoma cruzi parasites and T cells in intact, cleared organs and tissues. These techniques provide a reliable way to evaluate treatment outcomes and offer new insights into parasite-host interactions.
