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Immunology and Infection

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JoVE Journal Immunology and Infection
Quantitative 3D Imaging of Trypanosoma cruzi-Infected Cells, Dormant Amastigotes, and T Cells in Intact Clarified Organs
 

Quantitative 3D Imaging of Trypanosoma cruzi-Infected Cells, Dormant Amastigotes, and T Cells in Intact Clarified Organs

Article DOI: 10.3791/63919-v • 08:17 min • June 23rd, 2022
June 23rd, 2022 •
1, 1, 1, 1, 1,2
1Center for Tropical and Emerging Global Diseases, University of Georgia, 2Department of Cellular Biology, University of Georgia

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The present protocol describes light-sheet fluorescent microscopy and automated software-assisted methods to visualize and precisely quantify proliferating and dormant Trypanosoma cruzi parasites and T cells in intact, cleared organs and tissues. These techniques provide a reliable way to evaluate treatment outcomes and offer new insights into parasite-host interactions.

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Quantitative 3D Imaging Trypanosoma Cruzi-infected Cells Dormant Amastigotes T Cells Intact Clarified Organs Complete 3D Image Transparent Organs Quantification Replicating Parasites Dormant Parasites Immune Factor Cells Tissue Damage Epitope Immunostaining DNA Labeling Treatment Outcomes Mouse Models Chagas Disease Caleb Hawkins Research Technician Rick Tarleton's Lab Euthanizing Dissecting Fixing Organs CUBIC-L Solution
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