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Journal
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Immunology and Infection
/
结核分枝杆菌
通过尺寸排阻色谱实现细胞外囊泡富集
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Mycobacterium tuberculosis
Extracellular Vesicle Enrichment through Size Exclusion Chromatography
Please note that all translations are automatically generated.
Click here for the English version.
结核分枝杆菌
通过尺寸排阻色谱实现细胞外囊泡富集
DOI:
10.3791/63895-v
•
00:00 min
•
May 19, 2022
•
Joan M. Ryan
,
Karen M. Dobos
,
Nicole A. Kruh-Garcia
2
1
Mycobacteria Research Laboratories, Department of Microbiology Immunology & Pathology
,
Colorado State University
,
2
BioMARC, Infectious Disease Research Center
,
Colorado State University
Chapters
00:04
Introduction
00:50
Preparation of Crude Mtb EV Concentrate
02:36
Size Exclusion Chromatography for the Enrichment of Mtb EVs from CFP
05:52
Quantification and Qualification of the Mtb EVs
07:25
Results: Silver Stain and Western Blots by Fraction, TEM Images, and NTA Distribution
08:42
Conclusion
Summary
Automatic Translation
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Automatic Translation
该方案描述了尺寸排阻色谱,这是一种从培养物上清液中富集
结核分枝杆菌
细胞外囊泡的简单且可重复的技术。
Tags
Mycobacterium Tuberculosis
Extracellular Vesicles
Size Exclusion Chromatography
Enrichment
Ultracentrifugation
Centrifugal Filter
PBS
BCA Assay
Size Exclusion Chromatography Column
AFC Tower
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