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Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography
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Immunology and Infection
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JoVE Journal Immunology and Infection
Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography

Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography

English

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2,188 Views

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00:00 min

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May 19, 2022

DOI:

10.3791/63895-v

DOI:
10.3791/63895-v

•

00:00 min
May 19, 2022

•

5 Views
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1Mycobacteria Research Laboratories, Department of Microbiology Immunology & Pathology,Colorado State University, 2BioMARC, Infectious Disease Research Center,Colorado State University

Summary

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This protocol describes size exclusion chromatography, a facile and reproducible technique for enriching Mycobacterium tuberculosis extracellular vesicles from culture supernatants.

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Ryan, J. M., Dobos, K. M., Kruh-Garcia, N. A. Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography. J. Vis. Exp. (183), e63895, doi:10.3791/63895 (2022).

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