Journal
/
Inmunología y contagio
/
Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography
JoVE Revista
Inmunología y contagio
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Inmunología y contagio
Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography

Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography

2,188 Views

•

00:00 min

•

May 19, 2022

DOI:

10.3791/63895-v

DOI:
10.3791/63895-v

•

00:00 min
May 19, 2022

•

5 Views
, ,
1Mycobacteria Research Laboratories, Department of Microbiology Immunology & Pathology,Colorado State University, 2BioMARC, Infectious Disease Research Center,Colorado State University

Summary

Automatically generated

This protocol describes size exclusion chromatography, a facile and reproducible technique for enriching Mycobacterium tuberculosis extracellular vesicles from culture supernatants.

Explore More Videos

Mycobacterium TuberculosisExtracellular VesiclesSize Exclusion ChromatographyEnrichmentUltracentrifugationCentrifugal FilterPBSBCA AssaySize Exclusion Chromatography ColumnAFC Tower

Read Article

Cite this Article

Ryan, J. M., Dobos, K. M., Kruh-Garcia, N. A. Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography. J. Vis. Exp. (183), e63895, doi:10.3791/63895 (2022).

Download .ris file

Copy

Share Video

.

Copy