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年轻成人和老年沙鼠耳蜗的免疫标记和计数带状突触
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Immunolabeling and Counting Ribbon Synapses in Young Adult and Aged Gerbil Cochleae
DOI:

08:25 min

April 21, 2022

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Chapters

  • 00:05Introduction
  • 01:04Tissue Preparation
  • 02:21Fine Dissection of the Cochlea
  • 04:39Synapse Quantification
  • 06:52Results: Immunostaining of the Processed Cochleae
  • 07:50Conclusion

Summary

Automatic Translation

提出了一种通过免疫标记传入突触结构和毛细胞,在老年组织中淬灭自发荧光,解剖和估计耳蜗的长度以及量化通过共聚焦成像获得的图像堆栈中的突触来处理年轻成人和老年沙鼠耳蜗的方案。

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