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Immunolabeling and Counting Ribbon Synapses in Young Adult and Aged Gerbil Cochleae
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08:25 min
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April 21, 2022
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A protocol for processing young adult and aged gerbil cochleae by immunolabeling the afferent synaptic structures and hair cells, quenching autofluorescence in aged tissue, dissecting and estimating the length of the cochleae, and quantifying the synapses in image stacks obtained with confocal imaging is presented.
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Steenken, F., Bovee, S., Köppl, C. Immunolabeling and Counting Ribbon Synapses in Young Adult and Aged Gerbil Cochleae. J. Vis. Exp. (182), e63874, doi:10.3791/63874 (2022).
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