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Un approccio basato sulla spettrometria di massa per identificare le fosfatasi delle fosfoproteine e i loro interattori
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Biochemistry
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JoVE Journal Biochemistry
A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
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Un approccio basato sulla spettrometria di massa per identificare le fosfatasi delle fosfoproteine e i loro interattori

italiano

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2,203 Views

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10:17 min

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April 29, 2022

DOI:

10.3791/63805-v

DOI:
10.3791/63805-v

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10:17 min
April 29, 2022

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1 Views
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1Department of Biochemistry and Cell Biology,Geisel School of Medicine at Dartmouth, 2Norris Cotton Cancer Center

Summary

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Qui presentiamo un protocollo per l'arricchimento delle fosfatasi fosfoproteine endogene e delle loro proteine interagenti da cellule e tessuti e la loro identificazione e quantificazione mediante proteomica basata sulla spettrometria di massa.

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Cite this Article

Smolen, K. A., Kettenbach, A. N. A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors. J. Vis. Exp. (182), e63805, doi:10.3791/63805 (2022).

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