Here, we present a protocol for the enrichment of endogenous phosphoprotein phosphatases and their interacting proteins from cells and tissues and their identification and quantification by mass spectrometry-based proteomics.
Smolen, K. A., Kettenbach, A. N. A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors. J. Vis. Exp. (182), e63805, doi:10.3791/63805 (2022).