ジャーナル
/
生化学
/
A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
JoVE Journal
生化学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 生化学
A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

2,203 Views

•

10:17 min

•

April 29, 2022

DOI:

10.3791/63805-v

DOI:
10.3791/63805-v

•

10:17 min
April 29, 2022

•

1 Views
,
1Department of Biochemistry and Cell Biology,Geisel School of Medicine at Dartmouth, 2Norris Cotton Cancer Center

概要

Automatically generated

Here, we present a protocol for the enrichment of endogenous phosphoprotein phosphatases and their interacting proteins from cells and tissues and their identification and quantification by mass spectrometry-based proteomics.

Explore More Videos

Phosphoprotein PhosphatasesPIB-MSMass SpectrometryProteomicsPhosphatase InteractorsCell LysisProtein QuantificationPhosphatase Inhibitor ControlPhosphatase Inhibitor Beads

Read Article

Cite this Article

Smolen, K. A., Kettenbach, A. N. A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors. J. Vis. Exp. (182), e63805, doi:10.3791/63805 (2022).

Download .ris file

Copy

Share Video

.

Copy