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Durchflusszytometrische Analyse von Biomarkern zum Nachweis von Funktionsdefekten menschlicher Spermien
JoVE Journal
Medicine
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JoVE Journal Medicine
Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
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Durchflusszytometrische Analyse von Biomarkern zum Nachweis von Funktionsdefekten menschlicher Spermien

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1,594 Views

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08:48 min

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April 21, 2022

DOI:

10.3791/63790-v

DOI:
10.3791/63790-v

•

08:48 min
April 21, 2022

•

14 Views
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1Institute of Toxicology, College of Preventive Medicine,Army Medical University (Third Military Medical University), 2Department of Chemical Defense Medicine, College of Military Preventive Medicine,Army Medical University (Third Military Medical University)

Summary

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Das vorliegende Protokoll bietet eine praktische Lösung, die die Messung von Apoptose, mitochondrialem Membranpotenzial und DNA-Schäden in menschlichen Spermien mit einem einzigen Zytometer ermöglicht.

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Cite this Article

Ling, X., Zou, P., Ao, L., Zhou, N., Wang, X., Sun, L., Yang, H., Liu, J., Cao, J., Chen, Q. Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects. J. Vis. Exp. (182), e63790, doi:10.3791/63790 (2022).

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