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Microscopia a fluorescenza a riflessione interna simultanea e riflessione interna totale per l'imaging di microtubuli dinamici e proteine associate
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
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Microscopia a fluorescenza a riflessione interna simultanea e riflessione interna totale per l'imaging di microtubuli dinamici e proteine associate

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3,051 Views

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06:43 min

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May 03, 2022

DOI:

10.3791/63730-v

DOI:
10.3791/63730-v

•

06:43 min
May 03, 2022

•

11 Views
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1Department of Molecular Biophysics & Biochemistry,Yale University, 2Department of Physics,Yale University

Summary

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Presentiamo un protocollo per l'implementazione della microscopia a riflessione di interferenza e della microscopia a riflessione totale interna-fluorescenza per l'imaging simultaneo di microtubuli dinamici e proteine associate a microtubuli marcati fluorescenti.

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Tuna, Y., Al-Hiyasat, A., Howard, J. Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins. J. Vis. Exp. (183), e63730, doi:10.3791/63730 (2022).

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