Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Yazgan Tuna; Amer Al-Hiyasat; Jonathon Howard

doi:10.3791/63730

/

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

JoVE Journal
生化学

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JoVE Journal 生化学

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

3,050 Views

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06:43 min

•

May 03, 2022

DOI:

10.3791/63730-v

DOI:

10.3791/63730-v

•

06:43 min

May 03, 2022

•

11 Views

Yazgan Tuna¹, Amer Al-Hiyasat¹, Jonathon Howard^1,2

¹Department of Molecular Biophysics & Biochemistry,Yale University, ²物理学科,Yale University

概要

We present a protocol for implementing interference-reflection microscopy and total-internal-reflection-fluorescence microscopy for the simultaneous imaging of dynamic microtubules and fluorescently labeled microtubule-associated proteins.

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Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

概要

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