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Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

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06:43 min

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May 03, 2022

DOI:

10.3791/63730-v

DOI:
10.3791/63730-v

•

06:43 min
May 03, 2022

•

11 Views
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1Department of Molecular Biophysics & Biochemistry,Yale University, 2Department of Physics,Yale University

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We present a protocol for implementing interference-reflection microscopy and total-internal-reflection-fluorescence microscopy for the simultaneous imaging of dynamic microtubules and fluorescently labeled microtubule-associated proteins.

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Tuna, Y., Al-Hiyasat, A., Howard, J. Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins. J. Vis. Exp. (183), e63730, doi:10.3791/63730 (2022).

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