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沙 门氏菌 斑疹伤寒诱导的小鼠脓毒性腹膜炎的特征
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Immunology and Infection
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JoVE Journal Immunology and Infection
Characterizing Salmonella Typhimurium-induced Septic Peritonitis in Mice
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沙 门氏菌 斑疹伤寒诱导的小鼠脓毒性腹膜炎的特征

DOI:
10.3791/63695-v

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14:10 min

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July 29, 2022

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, , ,
1Department of Biochemistry,Indian Institute of Science, 2Center for BioSystems Science and Engineering,Indian Institute of Science, 3Undergraduate Program,Indian Institute of Science

Chapters

  • 00:03Introduction
  • 02:20Culture Preparation of Salmonella Typhimurium
  • 05:15Mice and Infections
  • 05:56CFU Assessment of Organs
  • 09:06Flow Cytometric Analysis of Various Immune Cell Populations in Peritoneal Exudate
  • 11:56Results
  • 13:22Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了小鼠模型系统中革兰氏阴性单细菌性脓毒症的诱导。该模型有助于研究脓毒症期间的炎症和致死宿主反应。

Tags

Salmonella TyphimuriumSeptic PeritonitisSepsisMice ModelGram-negative InfectionBacterial BurdenBSL-2LB BrothSalmonella Shigella Agar

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