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高解像度呼吸法による坐骨神経のミトコンドリア機能の評価
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Biochemistry
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JoVE Journal Biochemistry
Assessing Mitochondrial Function in Sciatic Nerve by High-Resolution Respirometry
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高解像度呼吸法による坐骨神経のミトコンドリア機能の評価

日本語

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2,118 Views

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08:19 min

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May 05, 2022

DOI:

10.3791/63690-v

DOI:
10.3791/63690-v

•

08:19 min
May 05, 2022

•

11 Views
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1Instituto de Bioquímica Médica Leopoldo de Meis, Centro de Ciências da Saúde,Universidade Federal do Rio de Janeiro

Summary

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蛍光センサーと組み合わせた高解像度の呼吸法は、ミトコンドリアの酸素消費量と活性酸素種(ROS)の生成を決定します。本プロトコールは、透過処理された坐骨神経におけるミトコンドリア呼吸数およびROS産生を評価する技術を記載する。

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Formiga-Jr, M. A., Camacho-Pereira, J. Assessing Mitochondrial Function in Sciatic Nerve by High-Resolution Respirometry. J. Vis. Exp. (183), e63690, doi:10.3791/63690 (2022).

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