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Padronização e Manutenção de Culturas Hepáticas Caninas 3D e Organoides Intestinais para Uso em Pesquisa Biomédica
JoVE Journal
Medicine
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JoVE Journal Medicine
Standardization and Maintenance of 3D Canine Hepatic and Intestinal Organoid Cultures for Use in Biomedical Research

Padronização e Manutenção de Culturas Hepáticas Caninas 3D e Organoides Intestinais para Uso em Pesquisa Biomédica

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4,038 Views

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08:39 min

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January 31, 2022

DOI:

10.3791/63515-v

DOI:
10.3791/63515-v

•

08:39 min
January 31, 2022

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10 Views
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1Department of Biomedical Sciences, College of Veterinary Medicine,Iowa State University, 2Department of Veterinary Clinical Sciences, College of Veterinary Medicine,Iowa State University, 33D Health Solutions Inc., 4Department of Molecular Virology and Microbiology,Baylor College of Medicine

Summary

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Métodos experimentais para colher células-tronco adultas de tecidos intestinais e hepáticos caninos para estabelecer culturas organoides 3D são descritos. Além disso, são discutidas as técnicas laboratoriais para garantir um crescimento consistente e fornecer procedimentos operacionais padrão para a colheita, biobanco e reviver culturas organoides caninas e hepáticas.

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Cite this Article

Gabriel, V., Zdyrski, C., Sahoo, D. K., Dao, K., Bourgois-Mochel, A., Kopper, J., Zeng, X., Estes, M. K., Mochel, J. P., Allenspach, K. Standardization and Maintenance of 3D Canine Hepatic and Intestinal Organoid Cultures for Use in Biomedical Research. J. Vis. Exp. (179), e63515, doi:10.3791/63515 (2022).

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