Dergi
/
Tıp
/
Standardization and Maintenance of 3D Canine Hepatic and Intestinal Organoid Cultures for Use in Biomedical Research
JoVE Journal
Tıp
This content is Free Access.
JoVE Journal Tıp
Standardization and Maintenance of 3D Canine Hepatic and Intestinal Organoid Cultures for Use in Biomedical Research

Standardization and Maintenance of 3D Canine Hepatic and Intestinal Organoid Cultures for Use in Biomedical Research

4,038 Views

•

08:39 min

•

January 31, 2022

DOI:

10.3791/63515-v

DOI:
10.3791/63515-v

•

08:39 min
January 31, 2022

•

10 Views
, , , , , , , , ,
1Department of Biomedical Sciences, College of Veterinary Medicine,Iowa State Üniversitesi, 2Department of Veterinary Clinical Sciences, College of Veterinary Medicine,Iowa State Üniversitesi, 33D Health Solutions Inc., 4Department of Molecular Virology and Microbiology,Baylor Tıp Fakültesi

Özet

Automatically generated

Experimental methods to harvest adult stem cells from canine intestinal and hepatic tissues to establish 3D organoid cultures are described. Furthermore, the laboratory techniques to ensure consistent growth and provide standard operating procedures to harvest, biobank, and revive canine intestinal and hepatic organoid cultures are discussed.

Explore More Videos

Canine Hepatic OrganoidsCanine Intestinal OrganoidsOrganoid CultureBiomedical ResearchStandardizationMaintenanceIsolationHarvestingBiobankingExtracellular MatrixChelating SolutionDMEMGrowth Factors

Read Article

Cite this Article

Gabriel, V., Zdyrski, C., Sahoo, D. K., Dao, K., Bourgois-Mochel, A., Kopper, J., Zeng, X., Estes, M. K., Mochel, J. P., Allenspach, K. Standardization and Maintenance of 3D Canine Hepatic and Intestinal Organoid Cultures for Use in Biomedical Research. J. Vis. Exp. (179), e63515, doi:10.3791/63515 (2022).

Download .ris file

Copy

Share Video

.

Copy