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Standardization and Maintenance of 3D Canine Hepatic and Intestinal Organoid Cultures for Use in Biomedical Research
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Medicina
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JoVE Revista Medicina
Standardization and Maintenance of 3D Canine Hepatic and Intestinal Organoid Cultures for Use in Biomedical Research

Standardization and Maintenance of 3D Canine Hepatic and Intestinal Organoid Cultures for Use in Biomedical Research

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08:39 min

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January 31, 2022

DOI:

10.3791/63515-v

DOI:
10.3791/63515-v

•

08:39 min
January 31, 2022

•

10 Views
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1Department of Biomedical Sciences, College of Veterinary Medicine,Iowa State University, 2Department of Veterinary Clinical Sciences, College of Veterinary Medicine,Iowa State University, 33D Health Solutions Inc., 4Department of Molecular Virology and Microbiology,Baylor College of Medicine

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Experimental methods to harvest adult stem cells from canine intestinal and hepatic tissues to establish 3D organoid cultures are described. Furthermore, the laboratory techniques to ensure consistent growth and provide standard operating procedures to harvest, biobank, and revive canine intestinal and hepatic organoid cultures are discussed.

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Canine Hepatic OrganoidsCanine Intestinal OrganoidsOrganoid CultureBiomedical ResearchStandardizationMaintenanceIsolationHarvestingBiobankingExtracellular MatrixChelating SolutionDMEMGrowth Factors

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Gabriel, V., Zdyrski, C., Sahoo, D. K., Dao, K., Bourgois-Mochel, A., Kopper, J., Zeng, X., Estes, M. K., Mochel, J. P., Allenspach, K. Standardization and Maintenance of 3D Canine Hepatic and Intestinal Organoid Cultures for Use in Biomedical Research. J. Vis. Exp. (179), e63515, doi:10.3791/63515 (2022).

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