Journal
/
Chemistry
/
Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
JoVE Journal
Chemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Chemistry
Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
Please note that all translations are automatically generated. Click here for the English version.

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

한국어

Automatically Generated

8,375 Views

•

11:12 min

•

February 07, 2022

DOI:

10.3791/63503-v

DOI:
10.3791/63503-v

•

11:12 min
February 07, 2022

•

80 Views
, , , , ,
1Department of Chemistry,Dalhousie University, 2Proteoform Scientific Inc.

Summary

Automatically generated

The present protocol describes solvent-based protein precipitation under controlled conditions for robust and rapid recovery and purification of proteome samples prior to mass spectrometry.

Explore More Videos

Protein PrecipitationOrganic SolventsMass SpectrometrySample PreparationProteome PurificationAcetoneSodium ChlorideMethanolChloroformCentrifugationSDSPelletVortex

Read Article

Cite this Article

Nickerson, J. L., Baghalabadi, V., Dang, Z., Miller, V. A., Little, S. L., Doucette, A. A. Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry. J. Vis. Exp. (180), e63503, doi:10.3791/63503 (2022).

Download .ris file

Copy

Share Video

.

Copy