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質量分析に先立つ堅牢なプロテオーム精製のための有機溶媒ベースのタンパク質沈殿
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Chemistry
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JoVE Journal Chemistry
Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
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質量分析に先立つ堅牢なプロテオーム精製のための有機溶媒ベースのタンパク質沈殿

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8,375 Views

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11:12 min

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February 07, 2022

DOI:

10.3791/63503-v

DOI:
10.3791/63503-v

•

11:12 min
February 07, 2022

•

80 Views
, , , , ,
1Department of Chemistry,Dalhousie University, 2Proteoform Scientific Inc.

Summary

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本プロトコールは、質量分析前のプロテオームサンプルの堅牢かつ迅速な回収および精製のための制御された条件下での溶媒ベースのタンパク質沈殿を記載する。

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Cite this Article

Nickerson, J. L., Baghalabadi, V., Dang, Z., Miller, V. A., Little, S. L., Doucette, A. A. Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry. J. Vis. Exp. (180), e63503, doi:10.3791/63503 (2022).

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