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Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
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JoVE Journal Chemistry
Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

English

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8,393 Views

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11:12 min

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February 07, 2022

DOI:

10.3791/63503-v

DOI:
10.3791/63503-v

•

11:12 min
February 07, 2022

•

80 Views
, , , , ,
1Department of Chemistry,Dalhousie University, 2Proteoform Scientific Inc.

Summary

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The present protocol describes solvent-based protein precipitation under controlled conditions for robust and rapid recovery and purification of proteome samples prior to mass spectrometry.

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Cite this Article

Nickerson, J. L., Baghalabadi, V., Dang, Z., Miller, V. A., Little, S. L., Doucette, A. A. Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry. J. Vis. Exp. (180), e63503, doi:10.3791/63503 (2022).

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