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09:30 min
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August 17, 2022
DOI:
1Centre for Women, Children, and Reproduction,Guangxi Key Laboratory of Metabolic Diseases Research, 2Department of Biopharmaceutics, School of Laboratory Medicine and Biotechnology,Southern Medical University, 3Department of Obstetrics and Gynecology,Chinese PLA General Hospital, 4Clinical Laboratory,Northern Theater Air Force Hospital, 5Guangzhou Darui Reproduction Technology Co., Ltd.
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このプロトコルは、半導体ベースの次世代シーケンシングプラットフォーム上での異数性に対する移植前遺伝子検査に必要なラボ内の全体的な手順を提示します。ここでは、全ゲノム増幅、DNA断片選択、ライブラリ構築、テンプレート調製、シーケンシング作業フローの詳細なステップを代表的な結果とともに紹介します。
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Xu, C., Wei, R., Lin, H., Deng, L., Wang, L., Li, D., Den, H., Qin, W., Wen, P., Liu, Y., Wu, Y., Ma, Q., Duan, J. Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform. J. Vis. Exp. (186), e63493, doi:10.3791/63493 (2022).
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