A subscription to JoVE is required to view this content. Sign in or start your free trial.
DNAファイバーアッセイを用いたヒト細胞における複製後ギャップの蓄積と修復の検出
Chapters
- 00:04Introduction
- 01:27S1 Fiber Experimental Setup
- 03:51DNA Fiber Preparation by Spreading
- 04:58Immunostaining of DNA Fibers
- 06:45Image Acquisition and Analysis
- 08:39Results: Post-Replicative ssDNA Gaps on Ongoing Forks by S1 Fiber Assay and Post-Replicative Repair by Gap Filling
- 09:42Conclusion
