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Limpieza óptica de tejidos vegetales para imágenes de fluorescencia
JoVE Journal
Biology
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JoVE Journal Biology
Optical Clearing of Plant Tissues for Fluorescence Imaging
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Limpieza óptica de tejidos vegetales para imágenes de fluorescencia

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4,430 Views

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04:55 min

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January 05, 2022

DOI:

10.3791/63428-v

DOI:
10.3791/63428-v

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04:55 min
January 05, 2022

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26 Views
, , , ,
1Institute of Transformative Bio-Molecules (ITbM),Nagoya University, 2Institute for Advanced Research (IAR),Nagoya University, 3Division of Biological Science, Graduate School of Science,Nagoya University, 4Department of Biological Sciences, Graduate School of Science,The University of Tokyo

Summary

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Aquí, se describe un método para hacer que los tejidos vegetales sean transparentes mientras se mantiene la estabilidad de las proteínas fluorescentes. Esta técnica facilita la obtención de imágenes profundas de tejidos vegetales despejados sin seccionamiento físico.

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Cite this Article

Kurihara, D., Mizuta, Y., Nagahara, S., Sato, Y., Higashiyama, T. Optical Clearing of Plant Tissues for Fluorescence Imaging. J. Vis. Exp. (179), e63428, doi:10.3791/63428 (2022).

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