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Optical Clearing of Plant Tissues for Fluorescence Imaging
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Optical Clearing of Plant Tissues for Fluorescence Imaging

Optical Clearing of Plant Tissues for Fluorescence Imaging

4,424 Views

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04:55 min

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January 05, 2022

DOI:

10.3791/63428-v

DOI:
10.3791/63428-v

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04:55 min
January 05, 2022

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26 Views
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1Institute of Transformative Bio-Molecules (ITbM),Nagoya University, 2Institute for Advanced Research (IAR),Nagoya University, 3Division of Biological Science, Graduate School of Science,Nagoya University, 4Department of Biological Sciences, Graduate School of Science,The University of Tokyo

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Here, a method is described for making plant tissues transparent while maintaining the stability of fluorescent proteins. This technique facilitates deep imaging of cleared plant tissues without physical sectioning.

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Kurihara, D., Mizuta, Y., Nagahara, S., Sato, Y., Higashiyama, T. Optical Clearing of Plant Tissues for Fluorescence Imaging. J. Vis. Exp. (179), e63428, doi:10.3791/63428 (2022).

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