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Limpeza Óptica de Tecidos Vegetais para Imagem de Fluorescência
JoVE Journal
Biology
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JoVE Journal Biology
Optical Clearing of Plant Tissues for Fluorescence Imaging

Limpeza Óptica de Tecidos Vegetais para Imagem de Fluorescência

português

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4,430 Views

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04:55 min

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January 05, 2022

DOI:

10.3791/63428-v

DOI:
10.3791/63428-v

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04:55 min
January 05, 2022

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26 Views
, , , ,
1Institute of Transformative Bio-Molecules (ITbM),Nagoya University, 2Institute for Advanced Research (IAR),Nagoya University, 3Division of Biological Science, Graduate School of Science,Nagoya University, 4Department of Biological Sciences, Graduate School of Science,The University of Tokyo

Summary

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Aqui, é descrito um método para tornar os tecidos vegetais transparentes, mantendo a estabilidade das proteínas fluorescentes. Esta técnica facilita a imagem profunda de tecidos vegetais limpos sem seção física.

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Cite this Article

Kurihara, D., Mizuta, Y., Nagahara, S., Sato, Y., Higashiyama, T. Optical Clearing of Plant Tissues for Fluorescence Imaging. J. Vis. Exp. (179), e63428, doi:10.3791/63428 (2022).

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