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将人 iPSC 稳健分化为纯脂肪细胞群以研究脂肪细胞相关疾病
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Developmental Biology
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JoVE Journal Developmental Biology
Robust Differentiation of Human iPSCs into a Pure Population of Adipocytes to Study Adipocyte-Associated Disorders
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将人 iPSC 稳健分化为纯脂肪细胞群以研究脂肪细胞相关疾病

DOI:
10.3791/63311-v

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10:31 min

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February 09, 2022

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, ,
1Diabetes Research Center, Qatar Biomedical Research Institute (QBRI),Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), 2College of Health and Life Sciences,Hamad Bin Khalifa University (HBKU), Qatar Foundation

Chapters

  • 00:05Introduction
  • 00:41Differentiation of Induced Pluripotent Stem Cells (iPSCs) into Mesenchymal Stem Cells (MSCs)
  • 05:12Differentiation of MSCs into Adipocytes and Sorting of Adipocytes Using Nile Red
  • 07:32Results: Generation of Pure Adipocytes from iPSCs
  • 09:21Conclusion

Summary

Automatic Translation

Automatic Translation

该方案允许从诱导多能干细胞（iPSC）产生纯脂肪细胞群。维甲酸用于将iPSCs分化为间充质干细胞（MSC），用于产生脂肪细胞。然后，采用基于尼罗河红染色的分选方法获得纯脂肪细胞。

Tags

Human IPSCsAdipocyte DifferentiationMesenchymal Stem CellsNile Red SortingMSC Differentiation MediaRetinoic AcidEmbryonic BodiesDMEM Low-glucose Medium

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