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Robust Differentiation of Human iPSCs into a Pure Population of Adipocytes to Study Adipocyte-Associated Disorders
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Developmental Biology
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JoVE Journal Developmental Biology
Robust Differentiation of Human iPSCs into a Pure Population of Adipocytes to Study Adipocyte-Associated Disorders

Robust Differentiation of Human iPSCs into a Pure Population of Adipocytes to Study Adipocyte-Associated Disorders

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2,604 Views

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10:31 min

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February 09, 2022

DOI:

10.3791/63311-v

DOI:
10.3791/63311-v

•

10:31 min
February 09, 2022

•

31 Views
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1Diabetes Research Center, Qatar Biomedical Research Institute (QBRI),Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), 2College of Health and Life Sciences,Hamad Bin Khalifa University (HBKU), Qatar Foundation

Summary

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The protocol allows the generation of a pure adipocyte population from induced pluripotent stem cells (iPSCs). Retinoic acid is used to differentiate iPSCs into mesenchymal stem cells (MSCs) which are used for producing adipocytes. Then, a sorting approach based on Nile red staining is used to obtain pure adipocytes.

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Human IPSCsAdipocyte DifferentiationMesenchymal Stem CellsNile Red SortingMSC Differentiation MediaRetinoic AcidEmbryonic BodiesDMEM Low-glucose Medium

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Cite this Article

Aghadi, M., Karam, M., Abdelalim, E. M. Robust Differentiation of Human iPSCs into a Pure Population of Adipocytes to Study Adipocyte-Associated Disorders. J. Vis. Exp. (180), e63311, doi:10.3791/63311 (2022).

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