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Neuroscience
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肌动蛋白动力学、离合器耦合和牵引力分析,促进生长锥体前进
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance
Please note that all translations are automatically generated.
Click here for the English version.
肌动蛋白动力学、离合器耦合和牵引力分析,促进生长锥体前进
DOI:
10.3791/63227-v
•
07:53 min
•
October 21, 2021
•
Takunori Minegishi
,
Ryosuke Fujikawa
,
Ria Fajarwati Kastian
,
Yuichi Sakumura
,
Naoyuki Inagaki
1
Laboratory of Systems Neurobiology and Medicine, Division of Biological Science
,
Nara Institute of Science and Technology
,
2
Laboratory of Data-Driven Biology, Division of Biological Science
,
Nara Institute of Science and Technology
Chapters
00:04
Introduction
00:35
Single Speckle Imaging at Neuronal Growth Cones
02:06
Traction Force Microscopy at Neuronal Growth Cones
03:46
Quantification of Traction Force
06:01
Results: Fluorescence Images and Force Mapping of a Neuronal Growth Cone
07:19
Conclusion
Summary
Automatic Translation
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Automatic Translation
为了进步,生长锥体必须对外部环境施加牵引力。牵引力的产生取决于肌动蛋白动力学和离合器联轴器。本研究描述了分析肌动蛋白动力学,离合器耦合和生长锥推进牵引力的方法。
Tags
Actin Dynamics
Clutch Coupling
Traction Force
Growth Cone Advance
Single Speckle Imaging
Traction Force Microscopy
TMR Ligand
Lifeact
HaloTag-actin
EGFP
SDS
Gel Substrate
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