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Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance

Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance

DOI:
10.3791/63227-v

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07:53 min

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October 21, 2021

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, , , ,
1Laboratory of Systems Neurobiology and Medicine, Division of Biological Science,Nara Institute of Science and Technology, 2Laboratory of Data-Driven Biology, Division of Biological Science,Nara Institute of Science and Technology

Capitoli

  • 00:04Introduction
  • 00:35Single Speckle Imaging at Neuronal Growth Cones
  • 02:06Traction Force Microscopy at Neuronal Growth Cones
  • 03:46Quantification of Traction Force
  • 06:01Results: Fluorescence Images and Force Mapping of a Neuronal Growth Cone
  • 07:19Conclusion

Summary

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Traduzione automatica

To advance, growth cones must exert traction forces against the external environment. The generation of traction forces is dependent on actin dynamics and clutch coupling. The present study describes methods for analyzing actin dynamics, clutch coupling and traction forces for growth cone advance.

Tags

Keywords: Actin DynamicsClutch CouplingTraction ForceGrowth Cone AdvanceSingle Speckle ImagingTraction Force MicroscopyTMR LigandLifeactHaloTag-actinEGFPSDSGel Substrate

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