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Murine Neural Plate Targeting door In Utero Nano-Injection (NEPTUNE) op Embryonale Dag 7.5
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February 14, 2022
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In dit protocol beschrijven we hoe de vruchtwaterholte van de muis op E7.5 kan worden geïnjecteerd met lentivirus, wat leidt tot uniforme transductie van de gehele neurale plaat, met minimale schadelijke effecten op overleving of embryonale ontwikkeling.
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Mangold, K., He, J., Stokman, S., Andersson, E. R. Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5. J. Vis. Exp. (180), e63148, doi:10.3791/63148 (2022).
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