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Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5
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Biología del desarrollo
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JoVE Revista Biología del desarrollo
Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5

Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5

DOI:
10.3791/63148-v

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10:49 min

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February 14, 2022

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, , ,
1Department of Cell and Molecular Biology,Karolinska Institutet

Capítulos

  • 00:05Introduction
  • 00:55Needle Loading
  • 01:38Injections
  • 07:53Results: Transduction of the Murine Neural Plate by Nano-Injection at Embryonic Day 7.5
  • 10:07Conclusion

Summary

Traducción Automática

Traducción Automática

In this protocol, we describe how to inject the mouse amniotic cavity at E7.5 with lentivirus, leading to uniform transduction of the entire neural plate, with minimal detrimental effects on survival or embryonic development.

Tags

NEPTUNEMurine Neural Plate TargetingIn Utero Nano-injectionEmbryonic Day 7.5Genetic ManipulationKnockdownLineage TracingShRNACdRNABarcoded Viral LibrariesNeurocienciasOrgan SystemsAmniotic CavityUltrasoundGlass NeedleMetal PlungerCapillary NeedleOilSolutionAnesthetized Female MousePainkillerChlorhexidine SolutionSkin IncisionMuscle LayerUterine HornsPBSElastic Membrane

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