Rivista
/
Biologia dello sviluppo
/
Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5
JoVE Journal
Biologia dello sviluppo
This content is Free Access.
JoVE Journal Biologia dello sviluppo
Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5

Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5

2,679 Views

•

10:49 min

•

February 14, 2022

DOI:

10.3791/63148-v

DOI:
10.3791/63148-v

•

10:49 min
February 14, 2022

•

3 Views
, , ,
1Department of Cell and Molecular Biology,Karolinska Institutet

Tags

NEPTUNEMurine Neural Plate TargetingIn Utero Nano-injectionEmbryonic Day 7.5Genetic ManipulationKnockdownLineage TracingShRNACdRNABarcoded Viral LibrariesNeuroscienzeOrgan SystemsAmniotic CavityUltrasoundGlass NeedleMetal PlungerCapillary NeedleOilSolutionAnesthetized Female MousePainkillerChlorhexidine SolutionSkin IncisionMuscle LayerUterine HornsPBSElastic Membrane

Read Article

Cite this Article

Mangold, K., He, J., Stokman, S., Andersson, E. R. Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5. J. Vis. Exp. (180), e63148, doi:10.3791/63148 (2022).

Download .ris file

Copy

Share Video

.

Copy