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次世代シーケンシングのためのマウス網膜内皮細胞の単離
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Developmental Biology
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JoVE Journal Developmental Biology
Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing
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次世代シーケンシングのためのマウス網膜内皮細胞の単離

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2,564 Views

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09:59 min

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October 11, 2021

DOI:

10.3791/63133-v

DOI:
10.3791/63133-v

•

09:59 min
October 11, 2021

•

6 Views
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1Department of Cell Biology,University of Virginia School of Medicine, 2Robert M. Berne Cardiovascular Research Center,University of Virginia School of Medicine, 3Department of Cardiology,University of Virginia School of Medicine, 4Hematovascular Biology Center,University of Virginia School of Medicine, 5Yale Cardiovascular Research Center,Yale University School of Medicine

Summary

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このプロトコルは、細胞収量、純度、および生存率に最適化されたマウス生後網膜内皮細胞の単離方法について説明しています。これらの細胞は、次世代シーケンシングアプローチに適しています。

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Cite this Article

Chavkin, N. W., Cain, S., Walsh, K., Hirschi, K. K. Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing. J. Vis. Exp. (176), e63133, doi:10.3791/63133 (2021).

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