/
/
Isolamento di cellule endoteliali retiniche murine per il sequenziamento di nuova generazione
A subscription to JoVE is required to view this content. Sign in or start your free trial.
2,564 Views
•
09:59 min
•
October 11, 2021
DOI:
1Department of Cell Biology,University of Virginia School of Medicine, 2Robert M. Berne Cardiovascular Research Center,University of Virginia School of Medicine, 3Department of Cardiology,University of Virginia School of Medicine, 4Hematovascular Biology Center,University of Virginia School of Medicine, 5Yale Cardiovascular Research Center,Yale University School of Medicine
Summary
Automatically generated
Questo protocollo descrive un metodo per l'isolamento di cellule endoteliali retiniche postnatali murine ottimizzate per la resa, la purezza e la vitalità delle cellule. Queste cellule sono adatte per approcci di sequenziamento di prossima generazione.
Read Article
Cite this Article
Chavkin, N. W., Cain, S., Walsh, K., Hirschi, K. K. Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing. J. Vis. Exp. (176), e63133, doi:10.3791/63133 (2021).
Copy