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Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing
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Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing
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09:59 min
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October 11, 2021
DOI:
1Department of Cell Biology,University of Virginia School of Medicine, 2Robert M. Berne Cardiovascular Research Center,University of Virginia School of Medicine, 3Department of Cardiology,University of Virginia School of Medicine, 4Hematovascular Biology Center,University of Virginia School of Medicine, 5Yale Cardiovascular Research Center,Yale University School of Medicine
Summary
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This protocol describes a method for the isolation of murine postnatal retinal endothelial cells optimized for cell yield, purity, and viability. These cells are suitable for next-generation sequencing approaches.
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Cite this Article
Chavkin, N. W., Cain, S., Walsh, K., Hirschi, K. K. Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing. J. Vis. Exp. (176), e63133, doi:10.3791/63133 (2021).
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