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Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
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Biochemistry
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JoVE Journal Biochemistry
Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

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2,294 Views

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11:44 min

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January 19, 2022

DOI:

10.3791/63118-v

DOI:
10.3791/63118-v

•

11:44 min
January 19, 2022

•

2 Views
, , , ,
1Department of Biological Sciences,The University of Alabama, 2Department of Pharmaceutical Botany, Faculty of Pharmacy,Hacettepe University

Summary

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The protocol describes the preparation of cell membrane affinity chromatography (CMAC) columns with immobilized cell membrane fragments containing functional transmembrane tropomyosin kinase receptor B proteins. The use of CMAC columns in the identification of specialized plant metabolites interacting with these receptors and present in complex natural mixtures is also explained.

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Arituluk, Z. C., Adhikari, B., Maitra, U., Goodman, C., Ciesla, L. M. Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B. J. Vis. Exp. (179), e63118, doi:10.3791/63118 (2022).

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