Journal
/
Bioquímica
/
Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
JoVE Journal
Bioquímica
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioquímica
Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

DOI:
10.3791/63118-v

•

11:44 min

•

January 19, 2022

•

, , , ,
1Department of Biological Sciences,The University of Alabama, 2Department of Pharmaceutical Botany, Faculty of Pharmacy,Hacettepe University

Capítulos

  • 00:05Introduction
  • 00:52Cell Homogenization
  • 03:56Cell Membrane Solubilization
  • 04:46Cell Membrane Immobilization on IAM.PC.DD2 Particles
  • 06:23CMAC Column Packing
  • 08:57Results: Antibody Staining and Frontal Affinity Chromatography of Immobilized TrkB Receptors
  • 11:02Conclusion

Summary

Tadução automática

Tadução automática

The protocol describes the preparation of cell membrane affinity chromatography (CMAC) columns with immobilized cell membrane fragments containing functional transmembrane tropomyosin kinase receptor B proteins. The use of CMAC columns in the identification of specialized plant metabolites interacting with these receptors and present in complex natural mixtures is also explained.

Tags

Keywords: Cellular Membrane Affinity ChromatographyTransmembrane ReceptorsPlant MetabolitesTropomyosin Kinase ReceptorBenzamidinePMSFProtease InhibitorATPTris-HCl BufferHomogenization BufferCell HarvestingCell LysisColumn Preparation

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/63118?article_type=v

Protein Complex Affinity Capture from Cryomilled Mammalian Cells
check_url/pt/63118?article_type=v

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
check_url/pt/63118?article_type=v

Assaying Protein Kinase Activity with Radiolabeled ATP
check_url/pt/63118?article_type=v

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
check_url/pt/63118?article_type=v

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
check_url/pt/63118?article_type=v

Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory
check_url/pt/63118?article_type=v

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
check_url/pt/63118?article_type=v

Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag
check_url/pt/63118?article_type=v

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
check_url/pt/63118?article_type=v

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Read Article