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Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
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Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
Capítulos
- 00:05Introduction
- 00:52Cell Homogenization
- 03:56Cell Membrane Solubilization
- 04:46Cell Membrane Immobilization on IAM.PC.DD2 Particles
- 06:23CMAC Column Packing
- 08:57Results: Antibody Staining and Frontal Affinity Chromatography of Immobilized TrkB Receptors
- 11:02Conclusion
The protocol describes the preparation of cell membrane affinity chromatography (CMAC) columns with immobilized cell membrane fragments containing functional transmembrane tropomyosin kinase receptor B proteins. The use of CMAC columns in the identification of specialized plant metabolites interacting with these receptors and present in complex natural mixtures is also explained.
