JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biochemistry
/
测量新鲜分离的小鼠肝细胞悬浮液中的脂肪酸β氧化
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biochemistry
Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes
Please note that all translations are automatically generated.
Click here for the English version.
测量新鲜分离的小鼠肝细胞悬浮液中的脂肪酸β氧化
DOI:
10.3791/62904-v
•
11:03 min
•
September 09, 2021
•
Schuyler D. Vickers*
1
,
Dominique C. Saporito*
1
,
Roberta Leonardi
1
Department of Biochemistry, School of Medicine
,
West Virginia University
Chapters
00:04
Introduction
00:56
Liver Perfusion and Dissociation
04:00
Fatty Acid β-oxidation Assay
08:59
Results: The Measurement of Fatty Acid β-Oxidation in a Mouse Hepatocytes Suspension Incubated with
14
C-Labeled Palmitic Acid
10:24
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
脂肪酸β氧化是负责在许多不同细胞类型(包括肝细胞)中产生能量的重要代谢途径。在这里,我们描述了一种使用
14
个C标记的棕榈酸测量新鲜分离的原代肝细胞中脂肪酸β氧化的方法。
Tags
Fatty Acid Beta-oxidation
Mouse Hepatocytes
Mitochondrial
Peroxisomal
Perfusion
Collagenase
M199 Buffer
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
脂肪酸鉴定<em>蜡状芽孢杆菌</em
镍 - 席夫碱的水解复杂环境条件适宜酸不稳定保护基团的保留
检测和可视化DNA损伤诱导的蛋白质复合物悬浮细胞培养使用邻近连接测定
小鼠肝灌注肝细胞和正弦内皮细胞的纯化
脂肪酸
13
C Isotopologue 分析提供了对无脊椎动物消费者营养碳转移和脂质代谢的洞察力
线粒体钙在分离线粒体和培养细胞中的流入分析
Langendorff 灌注后离体小鼠心脏 Polysomes 的动态蛋白质组学及 miRNA 分析
从茶中提取的新建细胞悬浮培养基中六种系统杀虫剂的代谢研究(
山茶西宁
西斯L.叶
从鸡蛋黄中分离脂蛋白颗粒,研究细菌病原体脂肪酸并入膜磷脂
使用核酸探针进行核酸活性的动力学筛选
Read Article