需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes
3,472 Views
•
11:03 min
•
September 09, 2021
DOI:
Summary
Automatically generated
Fatty acid β-oxidation is an essential metabolic pathway responsible for generating energy in many different cell types, including hepatocytes. Here, we describe a method to measure fatty acid β-oxidation in freshly isolated primary hepatocytes using 14C-labeled palmitic acid.
Read Article
Cite this Article
Vickers, S. D., Saporito, D. C., Leonardi, R. Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes. J. Vis. Exp. (175), e62904, doi:10.3791/62904 (2021).
Copy