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Clasificación celular activada por fluorescencia-tejido tratado con radioligando (FACS-RTT) para determinar el origen celular de la señal radiactiva
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September 10, 2021
DOI:
1Division of Adult Psychiatry, Department of Psychiatry,University Hospitals of Geneva, 2Department of Psychiatry,University of Geneva, 3Division of Nuclear medicine and Molecular Imaging,University Hospitals of Geneva, 4Division of Radiation Oncology, Department of Oncology,University Hospitals of Geneva, 5Department of Brain Sciences, Faculty of Medicine,Imperial College London
Summary
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Fluorescence-Activated Cell Sorting-Radioligand Treated Tissue (FACS-RTT) es una poderosa herramienta para estudiar el papel de la proteína translocadora de 18 kDa o la expresión del receptorserotonina 5HT 2A en la enfermedad de Alzheimer a escala celular. Este protocolo describe la aplicación ex-vivo de FACS-RTT en el modelo de rata TgF344-AD.
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Amossé, Q., Ceyzériat, K., Tsartsalis, S., Tournier, B. B., Millet, P. Fluorescence-Activated Cell Sorting-Radioligand Treated Tissue (FACS-RTT) to Determine the Cellular Origin of Radioactive Signal. J. Vis. Exp. (175), e62883, doi:10.3791/62883 (2021).
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